SANTA MONICA (CBSLA) – Biologists have confirmed that a young mountain lion living in the Santa Monica Mountains is the daughter of a cougar who was hit and killed by a car in January.

P-54 roams the Santa Monica Mountains in June 2018. (National Park Service)

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Biologists used DNA to prove that a mountain lion they recently tagged is P-54, the daughter of P-23, the National Park Service reported Thursday.

P-54, who is about 18 months old, was discovered in late February of 2017. P-54 is believed to be the product of inbreeding. Her mother is P-23 and her father is believed to be P-23’s half-brother, P-30.

A camera caught footage of P-54 that showed her “healthy and thriving,” the Santa Monica National Recreation Area wrote on Twitter.

In January, when P-54 was about a year old, her mother P-23 was struck and killed by a car on Malibu Canyon Road. At the time, biologists believed that P-54 would survive because most mountain lions leave their mothers between one and one-and-a-half years old.

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The region’s freeway network and shrinking habitat has served to isolate the mountain lion population in the Santa Monica Mountains, biologists say. That has led to frequent cases of inbreeding.

P-54 as a mountain lion cub in February 2017. (NPS)

P-23 was the 18th known case of a mountain lion killed on a freeway or road in the area since 2002, according to NPS officials.

In early March, the mountain lion known as P-64 completed a rare feet, crossed the 101 Freeway in the Liberty Canyon area of Agoura Hills. NPS researchers say the rare sighting highlights the area’s importance as an ideal location for a wildlife crossing.

Caltrans has proposed building a wildlife bridge across the 101 Freeway in Agoura Hills that would allow animals to travel between the Santa Monica Mountains and Simi Hills.

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The NPS has been studying mountain lion movements in the Santa Monica Mountains since 2002 using GPS collars.